This is a time-limited position that will last 18 months.
This position will be responsible for performing a variety of established laboratory procedures and techniques such as sequencing, confirmation and characterization of mutations in cell lines, high throughput genotyping of human DNA specimens, presentation of findings in meetings and conference calls with other consortium laboratories, preparation and submission of manuscripts for publication and maintenance of databases. This position also requires sequence and transcriptome analysis.
Perform experiments in a research lab focused on characterization of single nucleotide polymorphisms associated with asynchronous contralateral breast cancer. Studies include, but are not limited to: Fine mapping of disease associated regions by genotyping, isolation of DNA and RNA , preparation of cDNA, characterization of gene expression by RT- PCR , and preparation of PCR products for fragment analysis, sequencing, and cloning for functional analysis.
Perform experiments in the laboratory focused on the characterization of radiation sensitive cell lines by exome sequencing. Studies include, but are not limited to: confirmation of variants called from sequence data by PCR and sequencing in both genomic DNA and cDNA, characterization of gene expression by RT- PCR , and quantitation of protein production by Western blotting.
Data analysis. Evaluate putative genetic variants identified by sequence analysis and prioritize their laboratory study based upon bio-informatic analysis. Maintain a database of variants evaluated and their status. Work with collaborators to genetic association data from genomic regions associated with contralateral breast cancer.
Preparation of presentations and reports documenting results using spreadsheet, graphics, and word processing software. Make regular presentations at laboratory meetings and at meetings with collaborators both within UF and external.
Interact with external and internal collaborators. Must be able to communicate effectively with peers and faculty to accomplish research goals.
A bachelor’s degree in an appropriate area of specialization and one year of appropriate experience. Appropriate college coursework may substitute at an equivalent rate for the required experience.