The Senior Biological Scientist will support the Process Development (PD) laboratory to ensure that Florida Biologix at the Center of Excellence for Regenerative Health Biotechnology ( CERHB ) provides clients with new production methods and investigational materials for pre-clinical use. The incumbent will perform routine assays and testing including PCR , real time PCR , agarose gel electrophoresis, SDS - PAGE , western blot, immunocytochemistry and reporter gene based cell assays using insect or mammalian cell lines. The incumbent will design experiment-specific testing schemes to develop and validate assays or to establish assay positive controls. The incumbent will also troubleshoot and optimize assays under the supervision of the Research Coordinator (Upstream PD Manager). As needed, the incubent will be involved in researching and sourcing reagents, materials and equipment for the PD testing group. Functions will also involve maintaining the cell lines used for all cell based assays in the testing group ; ensuring that necessary positive controls are produced and validated ; that equipment are properly maintained and preventative maintenance is peformed at specify intervals; managing reagent and chemical supply chain for the PD testing laboratory ; ensuring that all equipment operate within their specification. This incumbent will also be expected to technically review data produced by self and others; to prepare assay development and/or assay optimization data for presentation; to participate in laboratory investigations, interpret data; and to write reports as needed. This position will assist in the planning and the coordination of upstream and downstream sample testing based on priority defined by the Research Coordinator. The selected candidate will be involved in the development of project specific timelines. The incumbent is also expected to create and modify documents (PDTRs and SOPs) and oversee new equipment installation. This position will work closely with Upstream and Downstream teams, Facilities and Quality Control units. This position could work collaboratively with clients and assists in transferring assays to the PD testing group.
Typically, Monday through Friday, 8:00 A.M. – 5:00 P.M.
Requires availability to work early in the morning, in the evenings, holidays, weekends and overtime when required to meet timelines.
Ability to stand for long periods. Ability to lift up to 25 lbs. Ability to maintain accurate records and to organize data. Flexibility, self-motivation, efficiency and responsibility are required for this position.
A bachelor’s degree in an appropriate area of specialization and three years of appropriate experience. Appropriate college coursework may substitute at an equivalent rate for the required experience.
Master’s and three years of work experience in the fields of cell biology, molecular biology, chemistry, biochemistry, molecular biology, biochemistry, and/or cell biology.
Hands on experience in cell culture, transfection, infection, reporter gene based infectious units titer assay; immunocytochemistry; rAAV production by transfection and by using baculovirus expression system. Hands on experience with nucleic-acid based, protein-based, cell-based and chemistry-based assays.
Working knowledge of modern laboratory procedures and biology techniques.
Familiarity with gene therapy vectors and knowledge of local, state and national regulations for working at the P1 and P2-levels of biological contaminant.
An in-depth understanding of upstream and good knowledge of downstream processing methods.
Experience in a cGMP production and testing environment.
Excellent communication skills including technical writing.
Health Assessment Required:
Special instructions to applicants:
Post offer health assessment is required for heavy lifting up to 25 lbs.
This is a time-limited position.
If you are a Florida resident claiming veteran’s preference, please upload a copy of your DD-214 with your application for consideration.