University of California, Davis - California

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Under general direction, responsible for performing genetic based testing necessary for identification and characterization of bacterial, protozoal and fungal isolates (PCR, DNA sequencing, DNA cloning). Assist with optimization and use of procedures for PCR-based (standard and real-time) differentiation and identification of bacterial, fungal and protozoal isolates and identification of virulence factors.

Use and help modify established procedures for amplifying the 16S rRNA gene and other relevant genes of uncultivable or difficult to isolate bacteria, fungi and protozoa directly from clinical samples. Organize and prioritize workload to meet the needs of these clinical testing requirements, maintain complete records on data collected, and assist in evaluating new procedures, organizing data for presentations and publications and performing literature searches.

Physical Demands:
Lift boxes and equipment weighing up to 30 lbs.

Ability to perform testing that require repetitive motions.

Work Environment:
  • Must be eligible for select agent FBI clearance.
  • Occasional overtime and share weekend/holiday coverage schedule.
  • Travel to CAHFS branch laboratories, conferences, etc.
  • Must be able to tolerate strong odors and the sight of exposed animal tissues and blood products. Must wear laboratory required and supplied uniform/protective clothing and participate in Health Surveillance Program. This position may, at times, require the incumbent to work with or be in areas where hazardous materials and/or infectious diseases are present. Specific hazards of the position are, but are not limited to rabies, chlamydia (psittacosis, ornithosis), salmonella, cryptosporidia.
  • This position is subject to Medical Surveillance procedures and review in accordance with Federal and State Laws and Regulations and University Policy.
Background Check Required:
This position is a critical position and subject to a background check. Employment is contingent upon successful completion of background investigation including criminal history and identity checks.



Minimum Qualifications:
  • Academic background and experience in molecular biology including theory and application. Knowledge in chemistry, biochemistry and biology.
  • Skills to extract and purify genomic and plasmid DNA from diagnostic specimens and/or non-ideal specimen types.
  • Experience with phenol chloroform DNA extraction.
  • Knowledge of and experience in molecular techniques including restriction endonuclease analysis, plasmid analysis, PCR and real-time PCR methodologies, DNA sequencing, and DNA cloning techniques.
  • Skills to operate a dissecting microscope, light microscope, spectrophotometer, digital imager, SmartCycler and/or other qPCR technologies, centrifuges, thermocyclers, and other laboratory equipment, including any accompanying software.
  • Ability to work in a laminar flow hood.
  • Experience working in a laboratory setting.
  • Writing skills to produce clear, concise report of test results and explain results to others.
  • Computer skills to use applications including electronic mail, word processing, spreadsheets, presentation graphics programs, library search programs, and graphing and statistical analysis programs.
  • Skills to use DNA sequencing and phylogenetic analysis programs such as NCBI, GenBank, and Vector NTI.
  • Organization and recordkeeping skills to meet workload requirements with close attention to detail.
  • Knowledge of quality control methods and their use in validating testing methodologies and interpretation of results.
  • Knowledge to design PCR primers, maintain stock, optimize concentrations, and perform all related calculations.
  • Ability to multi-task, work under pressure to meet deadlines, and adapt to sudden changes in workload.
  • Ability to apply scientific knowledge to solve complex problems.
Preferred Qualifications:
  • Experience working in a highly regulated environment such as ISO, AAVLD, or other laboratory accreditation standards.
  • Knowledge of and budgetary skills to manage and monitor appropriate and cost-effective ordering of laboratory reagents, supplies and equipment, and to project and justify expenditures for laboratory needs.
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University of California, Davis - 19 months ago - save job - block
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