Molecular Biologist
National Institutes of Health - Bethesda, MD

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Molecular Biologist
National Institute on Minority Health and Disparities, NIH, Bethesda, Maryland

The Applicant's Bachelor's degree (4-6 years' experience) or Master's degree (2-3 years' experience) must be in molecular biology, biochemistry or equivalent biological sciences degree from an accredited college or university with experience in wet-bench research. The Applicant will conduct wet-bench experiments on Human and animals cells centered on Cardiovascular Disease. Some of the duties will include:

  • Creating short oligonucleotide primers using Oligo programs for PCR and DNA sequencing.
  • Perform plasmid construction, amplification, and purification using digestion enzymes, bacterial cultures and the following plasmid purification kits: Qiagen, Eppendorf, and 5prime 3prime.
  • Perform mammalian cell culture transfection efficiency and promoter functionality (siRNA knockdowns) studies.
  • Use 14C labeled chloramphenicol acetyltransferase assay (CAT), beta galactosidase assay (Beta-GAL), and green florescent protein (GFP) methods to study TF bind and protein activity.
  • DNA, RNA and protein isolation using common methods such as sodium acetate/ethanol and chloroform/phenol DNA precipitation, Trizol RNA and Protein isolation methods, northern blotting, western blotting, SDS-PAGE, and agarose electrophoresis.
  • Familiar with most types of tissue culture to include DNA transfection of tissue culture cells using electroporation or lipofectamine TM .
To Apply:
Applicants should send curriculum vitae, a brief description of research interests, and three letters of reference to Dr. Adam R. Davis: adam.davis2@nih.gov

The NIH is dedicated to building a diverse community in its training and employment programs.
Home › career services › Jobs

Molecular Biologist
National Institute on Minority Health and Disparities, NIH, Bethesda, Maryland

The Applicant's Bachelor's degree (4-6 years' experience) or Master's degree (2-3 years' experience) must be in molecular biology, biochemistry or equivalent biological sciences degree from an accredited college or university with experience in wet-bench research. The Applicant will conduct wet-bench experiments on Human and animals cells centered on Cardiovascular Disease. Some of the duties will include:

  • Creating short oligonucleotide primers using Oligo programs for PCR and DNA sequencing.
  • Perform plasmid construction, amplification, and purification using digestion enzymes, bacterial cultures and the following plasmid purification kits: Qiagen, Eppendorf, and 5prime 3prime.
  • Perform mammalian cell culture transfection efficiency and promoter functionality (siRNA knockdowns) studies.
  • Use 14C labeled chloramphenicol acetyltransferase assay (CAT), beta galactosidase assay (Beta-GAL), and green florescent protein (GFP) methods to study TF bind and protein activity.
  • DNA, RNA and protein isolation using common methods such as sodium acetate/ethanol and chloroform/phenol DNA precipitation, Trizol RNA and Protein isolation methods, northern blotting, western blotting, SDS-PAGE, and agarose electrophoresis.
  • Familiar with most types of tissue culture to include DNA transfection of tissue culture cells using electroporation or lipofectamine TM .
To Apply:
Applicants should send curriculum vitae, a brief description of research interests, and three letters of reference to Dr. Adam R. Davis: adam.davis2@nih.gov

The NIH is dedicated to building a diverse community in its training and employment programs.

National Institutes of Health - 22 months ago - save job
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