Reports to the Principal Investigator. Responsibilities include designing, conducting, and analyzing scientific experiments in her/his field; presenting research results in oral and written formats; developing and maintaining knowledge of the current scientific literature. Operates highly independently. May supervise lower level project employees.
Job Description: Chondrocyte is the only cell type present in the cartilage and the response of chondrocytes to anabolic/catabolic stimuli is mediated by a wide variety of factors acting through specific receptors. Activation of these receptors leads, through cell signaling, transcriptional upregulation and post-transcriptional regulation, to a program of chondrocyte responses that determines the fate of the articular cartilage. Our laboratory is focused on identifying the novel transcriptional and post-transcriptional regulators of chondrocyte genes expression in diseased and healthy cartilage. Another area of interest is to identify and characterize active constituents in selected herbal extracts that have a long history of human use for the treatment of arthritis.
Will develop protocols and use high throughput genome scale technologies such as transcriptional profiling of coding and non-coding RNAs using microarrays, gene cloning, ChIP-Seq and deep sequencing of gene transcripts (RNASeq), Proteomics, LC/MS/MS, Preparative HPLC , animal models of arthritis, etc to broadly characterize the chondrocytic response to injury/inflammation and its modification by specific agents.
In consultation with the PI (a) To prepare and maintain primary chondrocytes and different cell lines, 3D culture of human chondrocytes, proliferation assay, FACS analysis. (b) Perform DNA and RNA extraction, ChIP- PCR , in vitro cRNA synthesis, cloning, quantitative real time and multiplex PCR , nuclear run off assay, Northern blot, Southern blot, ribonuclease protection assay, DNase hypersensitivity assay, siRNA experiments, Bioinformatic analysis of data, etc., © protein expression and purification, protein isolation, estimation and detection, Western blotting, co-immunoprecipitation, ELISA , luciferase assay, in vitro ubiquitination assay, GST pull down assay. (d) in situ hybridization, immunohistochemistry, Tunnel assay, H&E staining and immunocytochemistry. (e) handling and working with rodents (mice and rats), mice breeding and genotyping,etc. and other duties assigned from time-to-time.
The position requires M.D. or a Ph.D. in Life Sciences, Biochemistry or Molecular Biology from a recognized university with minimum of 3 years Post-Doctoral experience.
. Required qualifications plus a strong record of productive research in supported by publications in peer reviewed journals and a proven ability to work in a group setting. Applicants with bench expertise in cellular biochemistry, molecular biology, and, preferably miRNA and RNAi methodology, along with skills in data acquisition and statistical analysis are highly desirable and will be given preference.
NEOMED - 17 months ago