Cellular and Developmental Neurobiology, NINDS, NIH
Projects focus on:
Mechanisms underlying Neuronal Activity. Projects focus on the mechanisms underlying neuronal activity using GnRH neurons in vitro and in vivo. In vivo, GnRH neurons, spread throughout the forebrain, release GnRH in a pulsatile fashion to control reproduction. In explants, primary GnRH neurons continue to release in a pulsatile fashion and show synchronized calcium oscillations. Projects utilize the GnRH system to study molecular and cellular properties of neuronal activity (individual cells and neuronal population dynamics) at different developmental, metabolic and/or reproductive states. Candidate with experience in calcium imaging and/or electrophysiology preferred but not mandatory.
Recent publication: Bashour N, Wray S. Progesterone directly and rapidly inhibits GnRH neuronal activity via progesterone receptor membrane component 1, Endocrinology, 153: 4457-4469, 2012
Cell lineage, Neuronal Migration and Axonal Targeting . Projects focus on differentiation and migration of GnRH cells and olfactory axon outgrowth during development. Prenatally, GnRH neurons originate in the nasal region and migrate on olfactory axons into the brain. Current projects examine: 1) lineage of the GnRH cells, 2) intracellular signaling that controls cell movement and corresponding changes in cytoskeletal elements, and 3) combinatorial influence of guidance factors involved in migration of these cells into the forebrain; mechanisms common to neuronal migration as well as mechanisms specific to the GnRH system.
Recent publications: Forni et al., Neural Crest and Ectodermal Cells Intermix in the Nasal Placode to give rise to GnRH-1 Neurons, Sensory Neurons and Olfactory Ensheathing Cells, J. Neuroscience 31:6915-27, 2011; Casoni et al., SDF and GABA Interact to Regulate Axophilic Migration of GnRH-1 Neurons, Journal of Cell Science , 2012.
Laboratory uses multidisciplinary approaches: cre/lox mice, transgenic lines, imaging, nasal explants, videomicroscopy, calcium imaging, electrophysiology, immunocytochemistry, in situ histochemistry, single-cell PCR, and subtractive cDNA screening.
A fellow opening is available for either project.
NIH is an equal opportunity employer
Society for Neuroscience - 2 years ago