Responsible for assisting the principal investigator in the technical completion of cell culture and other closely-related experiments to mechanistically interrogate the regulation of BCL-2 family apoptotic regulators: implement native and denaturing poly-acrylamide gel electrophoresis (PAGE), quantitative polymerase chain reactions (qPCR), tissue culture of primary mouse bone marrow cells, isolation of primary mouse mitochondria, immunoblot analyses, and adeno-associated virus gene delivery to mice correctly with minimal supervision; assist in the development of experimental designs to test mitochondrial function of mouse mitochondria; modify or adapt existing or new protocols to determine the effects on electron transport chain function within the isolated mouse mitochondria as established by the principal investigator; and schedule work assignments for efficient and timely completion. Also responsible for performing documentation compilation and the reporting of experiments to the principal investigator: maintain accurate dated written laboratory notebook records; scan immunoblots; generate final digital images of results of biochemical analyses. Develop and archive protocols for isolating and analyzing primary mouse mitochondria. Reduce data (e.g. microscopy and immunoblots) into Adobe Photoshop, Adobe Illustrator and Microsoft PowerPoint as established by the principal investigator for publication and presentation. Present experimental data in a clear and concise manner to the principal investigator and department personnel. Prepare figures and author figure legends for the principal investigator to facilitate publication of data as requested or required. In addition, facilitate efficient laboratory operation: maintain up-to-date laboratory records (i.e. purchase orders, radiation safety reports, laboratory biohazards, chemical inventory, mouse database, frozen cell database, oligonucleotide orders, and hazardous material shipping information); prepare chemical reagents and solutions for isolating and analyzing animal mitochondria; monitor and maintain lab inventory of antibodies, oligonucleotides, plasmids and cells and other necessary supplies; maintain laboratory/departmental tissue culture incubators and freezers as required; demonstrate knowledge and execution of existing safety procedures including the proper disposal of hazardous waste as well as safe shipping practices for hazardous materials. Provide laboratory training and guidance to summer students, graduate students and post-doctoral fellows in various areas of scientific research.
A minimum of a Bachelor’s or foreign equivalent degree in an appropriate scientific field OR a Master’s or foreign equivalent degree (please see experience requirements).
If Bachelor’s degree, three (3) years of post-degree cancer molecular biology research experience performing cell culture-based experiments on human cancer cell lines; testing drugs on cancer cell lines and investigating chemoresistance; designing experiments and drafting protocols to study the role of nitric oxide and Protein Kinase G in preventing apoptosis in cancer cells is required. If Master’s degree, two (2) years of post-degree cancer molecular biology research experience performing cell culture-based experiments on human cancer cell lines; testing drugs on cancer cell lines and investigating chemoresistance; designing experiments and drafting protocols to study the role of nitric oxide and Protein Kinase G in preventing apoptosis in cancer cells is required.
This position also requires experience or background in each of the following:
conducting research projects with limited supervision and training;
analyzing scientific data utilizing data analysis software (Sigma Plot, GraphPad Prism, SPSS and Vector NTI);
mammalian cell cultures of various human cancers (lung, prostate, mesothelioma, breast cancer, melanoma and ovarian cancer cells);
performing various assays: cell culture-based assays (proliferation, cytotoxicity and cell death detection ELISA assays); radioimmunoassays; colony formation assays; and caspase activity assays;
performing SDS-PAGE and Western blotting while utilizing infrared imaging systems;
conducting ShRNA gene knockdown studies;
performing molecular cloning (restriction enzyme digestion analysis, primer designing, PCR, cloning of genes);
transient transfections of genes;
isolation of plasmid DNA and genomic DNA from cell lines; and
conducting DNA cell cycle analysis using Flow cytometry.
St. Jude Children's Research Hospital studies and treats catastrophic diseases in children, especially pediatric cancers. The hospital,...