Brigham and Women's Hospital (BWH) - Boston, MA

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Responsibilities: GENERAL SUMMARY: Working independently, under the general supervision of the Principal Investigator, and in accordance with established hospital policies and procedures, executes experiments involving in vitro and in vivo protocols and advanced molecular techniques. Participates in preclinical studies focused on the development and testing of novel targeted chemotherapies in ovarian cancer murine models and human tumor xenografts. Responsible for the orientation and training of new laboratory staff and interns. PRINCIPAL DUTIES AND RESPONSIBILITIES:
Under general supervision, performs a variety of routine tests following established methodologies.
Prepares and maintains tissue cultures.
Record data, summarizes, and analyzes results using basic statistics.
Prepares laboratory reagents, chemicals, instruments, and equipment.
Maintains supplies inventory in accordance with departmental procedures.
Independently conducts research protocols for the development, and analysis, and tumor imaging of mouse models of human ovarian cancer. Tasks include processing archival tissue blocks for histologic examination and conducting subsequent molecular analyses. Perform molecular biology procedures, such as DNA extraction, conventional PCR and real time PCR, and western blotting, and MTT assays,.
Establishes and maintains mouse colonies; performs survival surgery, microdissection and necropsy, and is involved in the delivery of conventional and novel targeted and nanoparticle-based chemotherapy drugs, following established protocols. In collaboration with the Principal Investigator, establishes new and modifies existing research methodologies and protocols.
Maintains tissue cultures and manipulates cell lines in a variety of experimental conditions. Monitors cells/cultures for viability and contamination. Perform cell viability MTT assays using various chemotherapy drugs. Perform lentiviral infection and immunoblotting of tissue cultures.
Calculates and analyzes data using advanced statistical techniques, prepares and helps prepare data and writes manuscripts for publication and grant applications.
Discusses progress of work regularly with Principal Investigator.
Maintains lab supply and reagent inventories and orders supplies and minor equipment.
Coordinates lab compliance with institutional policies and procedures in the areas of safety, environmental and infection control.
Assists in the orientation and training of new laboratory staff and interns.
Perform all other duties and responsibilities as directed, including both research and administrative duties.
Requirements: QUALIFICATIONS: Bachelor of Science or BABachelor of Arts in a biological science. At least 6 months experience in genetics, molecular/cellular biology techniques, and manipulation of animal models. Experience with histopahology and PCR techniques helpful. SKILLS/ABILITIES/COMPETENCIES REQUIRED:
Sound analytical and organizational skills
High degree of computer literacy.
Careful attention to detail.
Able to conduct in vivo experiments.
Excellent oral and written communication skills.
Must have sound interpersonal skills. SUPERVISORY RESPONSIBILITIES: Will participate in the orientation of new research staff. WORKING CONDITIONS: Normal basic science laboratory environment. Exposure to laboratory reagents, chemicals, adenoviruses and mouse tissues under controlled conditions. Minimal risk when established protocols are followed.
Shift: Day Shift
EEO Statement: An EEO, AA, VEVRAA Employer

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For over 150 years, Brigham and Women’s Hospital has been the birthplace of legendary medicine. A merger of three of Harvard’s...