HHMI is a major force in advancing biomedical research and science education in the U.S. and beyond. We have an opportunity for a Research Technician to join Mark J. Schnitzer’s laboratory at Stanford University in Palo Alto, CA. We are looking for a highly motivated molecular biology research technician who enjoys the excitement of a world-class scientific laboratory and welcomes the challenge of a fast paced environment that maintains a strong commitment to collegiality.
A major goal of the research in the lab is to advance understanding of normal cognitive and disease processes at the level of neural circuits, with emphasis on learning and memory processes. To achieve this goal the Schnitzer lab develops and applies novel in-vivo optical imaging techniques. The primary responsibilities of this position are to carry out recombinant DNA experiments, production and purifications of various viral vectors, animal care and genotyping, histology and immunochemistry, and lab management under the supervision of senior research staff. Other responsibilities include assisting with managing chemical inventory, viral vector database, safety records, and others that may not be mentioned above.
Principal Responsibilities:
Use molecular biology, cell culture, and histological techniques. Create and prepare new viral vectors to be used in labeling of neural cells in culture and/or in vivo.
Learn and conduct animal behavioral experiments
Perform surgical procedures, including 1) dissection of brain tissue from anesthetized or euthanized mice or rats for slice/ex vivo imaging procedures or histological staining; 2) various surgical procedures to prepare mice for live brain imaging procedures
Perform general lab maintenance and organization, ordering and stocking of supplies, preparing stock solutions following lab protocols.
Train new lab members regarding lab procedures and basic techniques.
Preferred Qualifications:
BA/BS in Biology, Cell Biology or Bioengineering required AND four or more years of related research experience. Advanced degree preferred.
Proficiency with the following techniques required: molecular cloning, PCR, cell culture techniques, transgenic colony maintenance, genotyping, and immuno-histochemistry.
Experience in various viral vectors including BSL2+ biological reagents required.
Experience in rodent surgeries.
Experience in protein engineering and purifications using HPLC and/or FPLC are desired.
General computer skills and ability to quickly learn and master computer programs.
Excellent organization and written/verbal communication skills, as well as willingness to learn and master new techniques, are a must.
Understanding of University policies and procedures as well as practices concerning laboratory safety, purchasing, and record retention.
Additional Information:
Please include the position title in the subject line. Submit 3 references with the CV.
Howard Hughes Medical Institute - 10 months ago
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