The Wyoming State Veterinary Laboratory (WSVL) in the Department of Veterinary Sciences is an accredited full-service veterinary diagnostic laboratory servicing veterinarians and animal owners in Wyoming and the surrounding region. The well-equipped histology unit is staffed by two histotechnologists and services four experienced board-certified veterinary pathologist.
The WSVL is located off-campus on Snowy Range Road.
Microtomy: Cutting diagnostic, wildlife and research blocks. Sections are routinely cut at 5 microns using a microtome. The section is placed on a slide and air-dried for a specific amount of time. H&E, Special Stains and IHC all require cutting slides.
H&E staining: Dried slides are stained using hematoxalyn and eosin. Tissue Tek autostainer is used regularly to do this. Staining may also be done by hand. Wildlife slides are air-dried overnight and stained the next morning. Large eyes are air-dried overnight; baked overnight and then stained and given out.
Coverslipping: After staining, slides are coverslipped in order to be viewed under a microscope by the pathologists. This is done using a case specific mounting medium and glass coverslips. It can be done using the Tissue Tek Glas coverslipper or by hand.
Immunohistochemistry: Slides are cut, dried overnight, baked overnight (or a minimum of 30 minutes); then deparaffinized. Slides are stained using 1 of 4 automatic stainers: Cadenza, Dako,Ventana NeXes or Benchmark XT. The antibody used designates which instrument to use. Each instrument has a specific protocol. Setup can include: reagent preparation, buffer preparation, antibody dilution, chromagen preparation, slide label preparation, and instrument programming. Once slides complete staining they are counterstained and coverslipped. Each instrument requires different daily, weekly, and monthly maintenance that must be tended to. Trouble shooting is also a part of IHC staining. New antibodies are worked up to establish them in the lab. Working up an antibody requires using different pretreatments, various blocks and antibody dilutions to get optimum staining.
Special Stains: Special stains are done at the request of the pathologist. Test tissues are cut onto slides with controls on them; dried for 10 to 20 minutes; baked for 30 minutes and then deparaffinized. Special stains are performed according to special stain protocols.
Embedding: This is done first thing in the morning, before others arrive. The person embedding takes the submitted piece(s) of tissue, orients it/them in a mold and makes a paraffin block. These are the blocks that are later cut to make slides for the pathologists to read. The embedder is also responsible for recording the number of cases and blocks in the Log Book and entering any pertinent information in the LIMS system. They also verify that the blocks received match the paper work.
Bachelor of Science degree in biological, chemical, physical, or clinical laboratory science from an accredited educational institution OR Histology Laboratory Technician (American Society for Clinical Pathology)-certified OR HTL registry-eligible as defined by the ASCP and one year experience in a laboratory including exposure to histo techniques.
Excellent interpersonal, written, and verbal communication skills.
Histotechnologist certification requirements.
Personal computer operation and applicable software applications.
Safety regulations, infection control and lab protocol procedures.
Work as a team member while meeting deadlines under pressure.
Operate and calibrate highly specialized and sensitive equipment.
Complete on-line application, resume, cover letter, and contact information for three work-related references.
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The University of Wyoming is located in Laramie, a town of 30,000 in the heart of the Rocky Mountain West. Wyoming is investing in its university, helping to make it a leader in academics, research and outreach.
The university has state-of-the-art facilities in many areas and is a cultural center for the state. Located on a high plain between the Laramie and Snowy Range mountain ranges, Laramie has more than 300 days of sunshine a year and is near year-round outdoor activities that include skiing, hiking, camping, bicycling, fishing and climbing.
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