2013 Janssen R&D Biologics Research Co-op April-September 2013 Job
Janssen Research & Development, LLC. (60 - Radnor, PA

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Radnor, PA, US2013 Janssen R&D Biologics Research Co-op April-September 2013(Job Number: 00000854)DescriptionJanssen Research & Development Pharmaceutical Research & Development, L.L.C., a member of the Johnson & Johnson Family of Companies, is recruiting for Biologics Research Co-ops to be based in Radnor, PA or Spring House, PA. The duration of this co-op is April to September 2013 (based on Drexel University's calendar).Janssen Research & Development (Johnson & Johnson) Pharmaceutical Research & Development, L.L.C., develops treatments that improve the health and lifestyles of people worldwide. Research and development areas encompass novel targets in neurologic disorders, gastroenterology, oncology, infectious disease, diabetes, hematology, metabolic disorders, immunologic disorders, and reproductive medicine.These roles are focused on specific project for students at the undergrad, Master's or PhD level. Here is a listing of our current availabilities: Project 1Biologics Research (BR) is increasingly getting involved in the development of bi-specific antibodies.

This is a new platform to BR and there are several questions that we have to address in the assay development arena. One of these questions is the determination of monovalent affinities for mono-specific and bi-specific candidates. Because of this, we anticipate that significant effort will be made to ensure that monomeric affinities to soluble and cell-surface expressed targets are obtained. This becomes more critical because some targets (e.g.

CD3) have limited solubility or cannot be expressed in soluble functional forms. This opportunity has a dual benefit for BR and a potential Co-op student. While the Co-op will benefit from learning technologies/methods that are applicable to biologics discovery in addition to exposure the biotherapeutic development, the Co-op will be a valuable asset providing laboratory support and intellectual input while working with a senior scientist.The candidate will conduct research within Biologics Research and Development. This requires attention to detail, understanding of concentration and related calculations and the preparation of samples using pipetting techniques.

The candidate will perform data analysis and reporting under senior scientist supervision: Apply standard laboratory procedures and operate basic laboratory equipment.Prepare/dilute samples to run characterization experimentsConduct experiments for methods development for/and affinity determination.Analysis of results using instrument software and spreadsheet data calculationsStore data in appropriate repositoriesDocument/report data in notebooks.Performs other duties as needed for every day lab operation.Project 2Development of rapid characterization tools of biologics using biochemical and chromatographic methodsa. Introduction - Janssen Biotechnology COE engineers biologics that comprise variable protein domains. The MCTD group of JBCOE requires orthogonal methods to evaluate conformational stability of a variety of biologics scaffolds.b. Goal - Hire a coop to develop tools to characterize biologics oxidation, deamidation, thiol content, and conformational changes using chromatography and gel based IP methods; fluorescence based AUC, and fluorescence dynamics in Radnor, PA.c.

Student type - senior undergraduate or graduate student to apply background in organic chemistry, analytical chemistry, physical chemistry, chemical engineering, materials science, and/or biochemistryThe Biophysics and Developability group of Janssen Biotechnology Center of Excellence in Radnor, PA. is looking for a coop student to join our team. This coop will work in a research laboratory using protein analytical chemistry, biochemical, and biophysical technologies. The coop will develop tools to characterize the oxidation, deamidation, thiol content, and conformational changes of biologics using chromatography and gel based IP methods; calorimetry, and fluorescence dynamics.

Responsibilities include: learning and applying laboratory skills in protein purification and protein biochemical and biophysical characterization; interpreting results; presenting written and oral presentations, and maintaining electronic records of experimental plans and results. The coop should be majoring in Chemical Engineering, Biophysics, Biochemistry, Life Science with lecture and laboratory course experience in Biology, Biochemistry, Molecular Biology, Mass Transfer/Heat Transfer, Separation Technologies, Chemistry, Biophysics, or related disciplines.Project 3Evolution has conserved a small number of receptors involved in receptor mediated transcytosis (RMT) that are found in the human gastrointestinal epithelium and vascular endothelium. These receptors help move proteins into tissue compartments to support their nutritional and growth requirements. RMT receptors transport molecules such as transferrin; EGF, insulin, IGF-I, hepatocyte growth factor, glucagon-like polypeptide receptor.

The purpose of this coop proposal is to determine the efficiency of selected RMT receptors in epithelial versus endothelial cells, where the same receptors are expressed. The student will be able to build on the knowledge around internalization demonstrated for cells within the group. The hypothesis to be tested is that the level of receptor expression and efficiency depends on the cell type. A number of complementary techniques will be used to compare this by combining functional pharmacology with High Content Imaging in a 96 well format.This position is for laboratory research using in vitro cellular biology techniques and high content imaging of RMT in epithelial and endothelial cells that endogenously express RMTs.

The selected student would learn functional imaging which pharmacological design of experiments and analysis of RMT combined with quantified image analysis to provide a multi-pronged approach to RMT efficiency of engineered peptides or proteins. They will have an ideal opportunity to train on state-of-the-art cell biology techniques and digital imaging.Specifically, the co-op will be expected to learn techniques of High Content Imaging and Analysis using cell and tissue culture expertise with an emphasis on appropriate conditions to determine receptor efficiency. The student will gain technical knowledge including microscopic digital imaging, immunhistochemistry, flow cytometry ELISA, Western, FACS analysis, RNA isolation and Taqman PCR. For this project, we are looking for candidate majoring in a life science (pharmacology, cell biology, physiology, biochemistry etc) and currently in a post-graduate program with prior experience working in a research lab.

Proficiency with computers, and software such as GraphPad Prism, Microsoft Word, Excel and PowerPoint are preferred. Good oral, written and interpersonal skills with the ability to document and effectively communicate interpretation of data and results. A student with cell biology experience and knowledge of imaging or pharmacology is preferred.QualificationsIn order to be considered for these positions, candidate must be enrolled in an accredited university pursuing an undergraduate, Master's or PhD degree. Students pursuing their undergraduate degree must have completed his/her freshman year, preferably in junior or senior standing.

Candidate must be pursuing technical, science based degree; examples include biology, biochemistry, molecular biology, cellular biology, chemistry, or related field. Lab experience strongly desired. Experience working independently and in a team research environment is preferred. Co-op must be available to work for 6 months from April to September 2013 and have the ability to work full-time during that time.Students must be able to provide their own transportation to/from their work location.

Students must be legally authorized to work in the United States and not require future sponsorship for employment visa status (e.g. H1-B status) now or in the future.BE VITAL in your career. Be seen for the talent you bring to your work. Explore opportunities within the Johnson & Johnson Family of Companies.Primary Location: North America-United States-Pennsylvania-RadnorOrganization: Janssen Research & Development, LLC.

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