The Moores Cancer Center is seeking a Laboratory Researcher to independently design, perform and analyze experiments to evaluate the roles of tight junction proteins in regulation of signal transduction molecules related to tumor growth, adhesion, metastasis, angiogenesis, and drug resistance. Design and perform in vivo experiments including primary tumor growth, tumor cell metastasis, spontaneous tumor models. Perform biochemical and molecular pharmacology and pharmacogenetic experiments with cancer drugs in vitro and in vivo including execution of basic and advanced biochemical and molecular biology assays.
Construction of vectors for the expression of recombinant tumor targeting toxins in E. coli, insect or mammalian cells, and production, purification and characterization of these novel protein therapeutics for testing their biologic activity using in vitro and in vivo tumor cell systems. Make significant innovative contributions pertaining to experimental design and methodology by reviewing published scientific data in refereed journals as well as online using search engines and subject-specific websites to make recommended changes and standards to current protocols and practices. Provide training and assistance to staff scientists, post-doctoral fellows and students.
Must be able to work occasional evenings or weekends as required.
Must be able to work with small animals and biohazardous materials.
Strong theoretical and technical knowledge of Molecular and Cellular Biology.
Experience analyzing and interpreting data. Experience designing new experiments as new techniques are established. Knowledge of and experience in testing research hypotheses.
Experience performing molecular and cellular immunology experiments including: DNA isolation, RNA isolation, ELISA assays, immunophenotyping, vector purifications, isolations and transfections, Western blotting, and the construction of vectors and preparation of plasmids and viruses.
Experience using immunophenotyping (cytometry) machines such as FACScan, viral transfection machine and incubators for tissue culture.
Knowledge of animal handling techniques as used for IV injections, acquisition of blood and tissue samples, euthanasia, and anesthesia.
- Expertise in aseptic surgical techniques in the capacity of researcher/teacher, to include organ manipulation, tissue explantations and proper use of anesthesia machines. *
Experience and knowledge in tumor analysis by immunohistochemistry; microscopy. Experience with performing tumor growth inhibition studies in mice including tumor inoculation and tumor measurements.
Theoretical and practical knowledge of the Xenogen IVIS 200 and OV100 live-imaging system.
Strong communication skills (verbal and written) needed to interact professionally and effectively in work environment. Effectively and accurately document research procedures. Ability to read, comprehend and discuss research materials.
University of California - San Diego - 2 years ago