MOLECULAR TECH II
Duke University - Durham, NC

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This is a Flow Cytometry Laboratory Position

Candidates must be certified Medical Technologists with Hematology experience. Preference given to those with Flow Cytometry Experience but not required.

Occupational Summary

Perform complex technical work involving molecular analysis of human biological material in a variety of specialized laboratories. Job duties will depend upon the specialty laboratory and may be composed of a combination of the following duties and responsibilities related to the specialization. Steps are extensive and complex requiring manual manipulation, close monitoring and control, extensive calculations, plus careful and accurate work. Training periods are long and will require reading literature related to the specialty to maintain current knowledge of these dynamic, rapidly evolving, fields.

Work Performed

LEVEL II PERSONNEL IN ALL LABORATORIES:

Perform complex technical work involved in preparing human biological material for specialized microscopic, cellular, serologic, molecular, genetic, and image or flow cytometric analysis. This may include any of the following operations, singly or in combination on submitted fluieds (including blood or body fluids), cytological specimens, or fixed and unfixed tissues: freeze,fix, dehydrate, embed, section, centrifuge, culture, harvest, cytospin, immunohistochemical
stain, immunofluorescent stain, hybridization of cytologic sections or suspensions and/or isolation of lymphocyte
subpopulations and/or genomic DNA.

Perform histocompatibility, cytogenetic, histologic, cytometric, immunohistochemical, immunofluorescent, serological, cellular, molecular, and/or cytologic procedures related to specialized microscopic, molecular, genetic, cellular, serological, electrophoretic, or cytometric analysis including assessing the quality of the sample for analysis.

Maintain files of any/all of the following as appropriate: slides, frozen specimens, blocks, cell preparations, micrographs, typing and antibody screening trays, DNA preparations, electrophoresis gel photos, results, and reports. Maintain neat and clean laboratory workspace.

Prepare stock solutions and reagents following standard operating procedures and quality control procedures. Recordquality control of reagent preparation. Recognize and resolve problems or report to upper level personnel.

Perform routine equipment maintenance according to standard operating procedures. Record temperatures and equipment maintenance in accordance with internal and external regulatory agencies. Recognize instrument malfunctions and report to upper level personnel.

Assist in inventory maintenance by monitoring inventory and notifying upper level personnel of low inventory.

Maintain logbook or database; accession samples,order tests, and bill cases. Interact with physicians, nurses, and other healthcare professionals.

Perform proficiency testing to maintain compliance with internal and external regulatory agencies.

Attend staff meetings, continuing education, safety and compliance training. Remain current on standard operating procedures by reviewing and signing the laboratory manual annually. Follow departmental and DUHS policies and procedures such as attendance and time reporting policies. Provide technical guidance, instruction, and consultation to faculty, students, and staff members as assigned including training of new personnel.

Operate complex and/or computerized microscopic, molecular, and/or cytometric equipment to analyze
prepared cellular and molecular specimens, calibrate and maintain instrumentation, and validate quality of assay.

Type results, reports, and addendums to reports and/or enter preliminary results into Cerner including making case specific comments pertaining toquality of specimens and/or interpretation of results.

Assist in the development of clinical assays and the validation of those assays including performance of testing and/or analysis.

Perform other related duties incidental to the work described herein.

LEVEL II PERSONNEL MAY BE REQUIRED TO PERFORM ONE OR MORE OF THE FOLLOWING DUTIES DEPENDING UPON SPECIALTY LABORATORY:

Molecular Diagnostics and Histocompatibility Laboratories: Extract nucleic acids, set up PCR and digestion, prepare and run electrophoretic gels, transfer and hybridize including the preparation of oligonucleotide probes.

Cytogenetics and Image Cytometry Laboratories: Perform fluorescence in situ hybridization utilizing multi-color probes
and microscopic analysis using specialized microscopic equipment and software. Perform cytometric or karyotypic analysis of stained cytologic sections or suspensions using specialized microscopic and/or cytometric equipment.

Immunopathology and Flow Cytometry Laboratories: Perform direct immunofluorescent staining of cell suspensions or frozen sections for the purpose of diagnosing malignant diseases as in lymphoma and leukemia immunophenotyping.

Flow Cytometry Laboratory: Perform direct immunofluorescentstaining of lymphocytes for the purpose of monitoring immune compromised patients by flow cytometric analysis.

Histocompatibility, Molecular Diagnostics, andImmunopathology Laboratories: Perform histocompatibility testing using serological, cellular, molecular, electrophoretic, and flow cytometric methodologies,including assessing the quality of the sample for analysis. Perform transplant donor and recipient, pre- andpost-transplantation analysis to determine
eligibility for transplantation or to determine acceptance or rejection of transplantation by any of the following methods: HLA typing, engraftment-PCR analysis, myeloid/lymphoid fractionation, immunofluorescence staining, or flow cytometric analysis.

Electron Microscopy Laboratory: Perform specialized microscopic analysis of subcellular components and identify viral particles in samples prepared using histologic or cytologic procedures designed for electron microscopy.

Knowledge, Skills and Abilities
Must be trained in at least half of

Minimum Qualifications
Education

BS degree in Biological Science or related field is required for the Histocompatibility, Flow Cytometry, Cytogenetics, and Molecular Diagnostics Laboratories. A BS degree in Biological Science or related field is preferred for the Immunopathology, Cytogenetics, and Image Cytometry Laboratories. Those people without BS degrees who are working at this level in Immunopathology, Cytogenetics, Electron Microscopy, or Image Cytometry as of December 31, 2004 will be grandfathered. Experience

Two years experience in specialty laboratory or 3 years of general laboratory experience in addition to certification with a minimum of one year with relevant experience of specialty type. Degrees, Licensures, Certifications

Certification in one of the following is required at this level depending upon the specialty laboratory in which the person is employed: Image Cytometry and Immunopathology: HTL(ASCP) or HT(ASCP) Flow Cytometry: MT(ASCP) or MT(ASCP) specialty Cytogenetics: CLSp(CG)-NCA Molecular Diagnostics: CLSp(MB)-NCA Clinical Transplant Immunology: CHT(ABHI), additional MT(ASCP) preferred Electron Microscopy: not required, HTL(ASCP) or HT(ASCP

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