A Research Assistant II opportunity is available to join the Conrad Prebys Center for Chemical Genomics (CPCCG) and contribute toward the Center’s chemical biology and drug discovery efforts at the La Jolla campus.
Will join a collaborative team to perform tissue culture, molecular biology techniques and protein analysis necessary for cell based and biochemical screening, as well as perform a variety of other related duties and tasks as required or assigned.
Under specific direction, will operate and maintain state-of-the-art laboratory and scientific equipment as needed to identify technical problems and opportunities and interpret manufacturer manuals and resources to fully-utilize equipment. Will also gain technical knowledge and skills to develop novel cell based and biochemical assays which will be converted to miniaturize 384-well/1536-well format using automated liquid handlers and plate readers.
Requires a bachelor’s degree in a biological science, chemistry, physics or a related field and a minimum of 1-3 years of directly related experience in tissue culture, or an equivalent combination of education, training and experience from which comparable knowledge, skills and abilities have been attained. Candidates with a master’s degree, a minimum of 1 year are required.
Must have proven experience concurrently maintaining multiple (approx. 5-10) mammalian cell lines, the ability to troubleshoot and solve/modify standard procedures as needed with input from supervisor and colleagues, and prior experience with acquisition and analysis of multi-parametric quantitative experimental data.
The Research Assistant II must have demonstrated previous experience and knowledge with mammalian cell culture, basic molecular biology experience and protein analysis techniques, such as PCR, plasmid purification, agarose and polyacrylamide gel electrophoresis and restriction digests.
Requires the ability to work independently as well as a demonstrated ability to work in a highly interactive team within a matrix environment and the ability manage multiple projects in a rapidly changing, deadline-driven environment. Must also be able to interpret, evaluate, analyze and present data, and to accurately document and record experiments in lab notebooks.
Must have a professional demeanor and excellent verbal, written, interpersonal and organizational skills. Must have professional proficiency in Microsoft Office (particularly Excel) and the ability to learn department-specific computer systems as needed.
Prior experience with Prism software, cell-based high-content screening, si/shRNA, automated multi-well plate preparation experience and experience with a wide-range of liquid-handling instruments is strongly desired.