Research Technician - Structural Biology
Memorial Sloan-Kettering Cancer Center - New York, NY

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We are looking for a Research Technician to provide technical support for the Structural Biology lab. The lab is interested in developing a new generation of optical imaging technologies to enable advances in two hitherto difficult-to-investigate areas: the real-time analysis of macromolecular interactions and motions at the nanometer scale in vivo and the three-dimensional architecture of complex molecular machines and of subcellular ultrastructure, in situ.

Responsibilities include, but are not limited to:
•Preparation of mammalian nuclear factors, including recombinant proteins produced in bacteria and insect cells, as well as native large macromolecular complexes from human cells
•Assay purified proteins using in vitro transcription assays, native gel electrophoresis, DNAse I and permanganate footprinting
•Design, clone, and assay constructs for various genes of interest using standard molecular and cell biology techniques (PCR, transformation, cloning, subcloning, DNA/RNA miniprep and maxiprep, Western and Northern blotting, etc.)
•Responsible for cell culture procedures, including large-scale growth of mammalian cells in suspension and of mouse embryonic stem cells
•Ensure stocks of lab supplies are maintained, place orders as necessary, maintain lab equipment and perform other duties as assigned
•BA/BS in biological sciences with a minimum of (1) to three (3) years research experience. MS degree preferred.
•Strong technical ability in molecular/cell biology and biochemistry required
•Good verbal and written communication, interpersonal, and computer skills required
•Familiarity with gene targeting techniques, preferably in mice
•Previous protein purification experience preferably with endogenous nuclear complexes
•Ability to be organized, dependable, efficient, and to work independently once fully trained
MSKCC is an equal opportunity and affirmative action employer committed to diversity and inclusion in all aspects of recruiting and employment. All qualified individuals are encouraged to apply and will receive consideration without regard to race, color, gender, gender identity or expression, sexual orientation, national origin, age, religion, creed, disability, veteran status or any other factor which cannot lawfully be used as a basis for an employment decision.

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Memorial Sloan Kettering Cancer Center has devoted over a century to exceptional patient care, innovative research, and outstanding...