As a lab technician assigned to create a cysteine dioxygenase mutant I was primarily involved in generating the mutant as well as confirming the correct sequence for the generated mutant DNA. My direct superior was both informative as well as understanding, which to me created an ideal work environment. The procedure to generate the CDO mutants required optimization in order to work correctly. The most frustrating and difficult part of this assignment was the troubleshooting and optimization of the protocol. However, the sense of accomplishment garnered when success was attained more than made up for any frustration. I would gladly continue work in this setting were it a possibility.